Immunological cross-reaction between sperm dynein heavy chains from different species

Immunological cross-reaction between sperm dynein heavy chains from different species.

The 19S outer arm dynein of trout sperm flagella is a complex of proteins composed of two heavy chains, five intermediate chains and at least six light chains. After dialysis against a low ionic strength buffer, its beta subunit was purified and used to generate a rabbit polyclonal antibody. This polyclonal antibody reacted strongly with the trout beta dynein heavy chain (DHC) but not with the ...

Isolation and characterization of a novel dynein that contains C and A heavy chains from sea urchin sperm flagellar axonemes.

A novel dynein (C/A dynein), which is composed of C and A heavy chains, two intermediate chains and several light chains, was isolated from sea urchin sperm flagella. The C/A dynein was released by the treatment with 0.7 M NaCl plus 5 mM ATP from the axonemes depleted of outer arm 21 S dynein. Sedimentation coefficient of this dynein was estimated by sucrose density gradient centrifugation to b...

Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles

We describe two dynein heavy chain (DHC)-like polypeptides (DHCs 2 and 3) that are distinct from the heavy chain of conventional cytoplasmic dynein (DHC1) but are expressed in a variety of mammalian cells that lack axonemes. DHC2 is a distant member of the "cytoplasmic" branch of the dynein phylogenetic tree, while DHC3 shares more sequence similarity with dynein-like polypeptides that have bee...

Chymotryptic digestion of Tetrahymena ciliary dynein. II. Pathway of the degradation of 22S dynein heavy chains

As shown in the preceding paper (Toyoshima, Y. Y., 1987, J. Cell Biol., 105:887-895) three-headed Tetrahymena 22S dynein consists of three heavy chains (HCs) and is decomposed into two-headed (H) and one-headed (L) fragments by chymotryptic digestion. To accurately determine the presence of multiple ATPases and ultimately the location of various domains, it is necessary to determine the identit...

Different MHC Class I Heavy Chains